Human Allergin-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7638
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 767727
Product Summary for Human Allergin-1 Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Allergin-1
Met1-Lys227
Accession # Q7Z6M3
Met1-Lys227
Accession # Q7Z6M3
Specificity
Detects human Allergin-1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Allergin-1 Antibody
Detection of Allergin‑1 in Human Blood Monocytes by Flow Cytometry.
Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3832F) and either (A) Mouse Anti-Human Allergin-1 Monoclonal Antibody (Catalog # MAB7638) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Allergin‑1 in U‑87 MG Human Cell Line.
Allergin-1 was detected in immersion fixed U-87 MG human glioblastoma/astrocytoma cell line using Mouse Anti-Human Allergin-1 Monoclonal Antibody (Catalog # MAB7638) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Allergin-1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood monocytes
Sample: Human peripheral blood monocytes
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed U‑87 MG human glioblastoma/astrocytoma cell line
Sample: Immersion fixed U‑87 MG human glioblastoma/astrocytoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Allergin-1
MCA32 (Mast Cell Antigen; also Allergin‑1 and c17orf60) is a 70 kDa member of the Ig Superfamily. It is expressed on mast cells, basophils, neutrophils and macrophages, and is reported to negatively regulate allergic mediator release from mast cells. In particular, MCA32 interacts with Fc epsilonRI. Upon IgE mediated Fc epsilonRI aggregation and activation, Allergin‑1 recruits Tyr phosphatases to a Fc epsilonRI tetrameric complex, dampening IgE receptor downstream signaling. Mature human Allergin‑1 is a monomeric type I transmembrane glycoprotein that is 324 amino acids (aa) in length. It contains two C2-type Ig domains (aa 35-118 and 123-217) in the extracellular region (aa 20-227), plus a 95 aa cytoplasmic domain that possesses two ITIM motifs. Two 40 kDa splice variants exist that show deletions of the Ig-like domains. One isoform termed Allergin‑1S1 is missing aa 128-217, while a second isoform called Allergin‑1S2 is missing aa 33-122. This second isoform represents the antigen used for immunization in this product (GenBank BAJ08253). Over aa 29-131, the Allergin‑1S2 isoform shares 100% sequence identity with human Allergin‑1L full length isoform and only 24% aa sequence identity with mouse Allergin‑1.
Long Name
Allergy Inhibitory Receptor 1
Alternate Names
Allergin1, C17orf60, MCA32, MCA32-1S2, MILR1
Gene Symbol
MILR1
UniProt
Additional Allergin-1 Products
Product Documents for Human Allergin-1 Antibody
Product Specific Notices for Human Allergin-1 Antibody
For research use only
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