Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human a-1 Adrenergic R/ADRA1A. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human a-1 Adrenergic R/ADRA1A Monoclonal Antibody (Catalog # MAB10298) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-1 Adrenergic R/ADRA1A at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of alpha‑1 Adrenergic R/ADRA1A in HEK293 Human Cell Line Transfected with Human alpha‑1 Adrenergic R/ADRA1A and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human a-1 Adrenergic R/ADRA1A or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human a-1 Adrenergic R/ADRA1A Monoclonal Antibody (Catalog # MAB10298) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Applications for Human alpha-1 Adrenergic R/ADRA1A Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human alpha‑1 Adrenergic R/ADRA1A and eGFP

Western Blot

2 µg/mL
Sample: HEK293T human embryonic kidney cell line transfected with human alpha‑1 Adrenergic R/ADRA1A

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-1A Adrenergic R/ADRA1A

ADRA1A (formerly known as alpha 1-C adrenergic receptor) is an alpha-adrenergic receptor which is mediated by G(q) and G(11) proteins. Alpha-1 Adrenoreceptors play an important role in mediating human prostatic smooth muscle contraction. Antagonists of ADRA1A include tamsulosin and silodosin which treat benign prostatic hyperplasia.

Long Name

Alpha-1A Adrenergic Receptor

Alternate Names

a-1A AdrenergicR, ADRA1A, ADRA1L1, alpha1A AdrenergicR, ALPHA1AAR

Entrez Gene IDs

148 (Human); 1549 (Mouse); 29412 (Rat)

Gene Symbol

ADRA1A

UniProt

P35348

Additional alpha-1A Adrenergic R/ADRA1A Products

Product Documents for Human alpha-1 Adrenergic R/ADRA1A Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human alpha-1 Adrenergic R/ADRA1A Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human alpha-1 Adrenergic R/ADRA1A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10298

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all alpha-1A Adrenergic R/ADRA1A Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human alpha-1 Adrenergic R/ADRA1A Antibody

Detection of Human alpha‑1 Adrenergic R/ADRA1A by Western Blot.