Human alpha-1 Adrenergic R/ADRA1A Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10298
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 518508
Product Specifications
Immunogen
HEK293 human embryonic kidney cell line transfected with human alpha‑1 Adrenergic R/ADRA1A
Accession # P35348
Accession # P35348
Specificity
Detects human alpha‑1 Adrenergic R/ADRA1A in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human alpha-1 Adrenergic R/ADRA1A Antibody
Detection of Human alpha‑1 Adrenergic R/ADRA1A by Western Blot.
Western blot shows lysates of HEK293T human embryonic kidney cell line either mock transfected or transfected with human a-1 Adrenergic R/ADRA1A. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human a-1 Adrenergic R/ADRA1A Monoclonal Antibody (Catalog # MAB10298) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-1 Adrenergic R/ADRA1A at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of alpha‑1 Adrenergic R/ADRA1A in HEK293 Human Cell Line Transfected with Human alpha‑1 Adrenergic R/ADRA1A and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human a-1 Adrenergic R/ADRA1A or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human a-1 Adrenergic R/ADRA1A Monoclonal Antibody (Catalog # MAB10298) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.Applications for Human alpha-1 Adrenergic R/ADRA1A Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human alpha‑1 Adrenergic R/ADRA1A and eGFP
Sample: HEK293 Human Cell Line Transfected with Human alpha‑1 Adrenergic R/ADRA1A and eGFP
Western Blot
2 µg/mL
Sample: HEK293T human embryonic kidney cell line transfected with human alpha‑1 Adrenergic R/ADRA1A
Sample: HEK293T human embryonic kidney cell line transfected with human alpha‑1 Adrenergic R/ADRA1A
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-1A Adrenergic R/ADRA1A
Long Name
Alpha-1A Adrenergic Receptor
Alternate Names
a-1A AdrenergicR, ADRA1A, ADRA1L1, alpha1A AdrenergicR, ALPHA1AAR
Gene Symbol
ADRA1A
UniProt
Additional alpha-1A Adrenergic R/ADRA1A Products
Product Documents for Human alpha-1 Adrenergic R/ADRA1A Antibody
Product Specific Notices for Human alpha-1 Adrenergic R/ADRA1A Antibody
For research use only
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