HEK293 human embryonic kidney cell line transfected with either (A) human ADRA2A or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human ADRA2A Monoclonal Antibody (Catalog # MAB10129) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Applications for Human alpha-2A Adrenergic R/ADRA2A Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human ADRA2A and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-2A Adrenergic R/ADRA2A

Alpha-2-adrenergic receptors, including ADRA2A, are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mouse revealed that both ADRA2A and ADRA2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons. The ADRA2A subtype inhibited transmitter release at high stimulation frequencies, whereas the ADRA2C subtype modulated neurotransmission at lower levels of nerve activity.

Long Name

Alpha-2A Adrenergic Receptor

Alternate Names

a-2A AdrenergicR, ADRA2A, alpha2A AdrenergicR, ZNF32

Entrez Gene IDs

150 (Human); 11551 (Mouse); 25083 (Rat)

Gene Symbol

ADRA2A

Additional alpha-2A Adrenergic R/ADRA2A Products

Product Documents for Human alpha-2A Adrenergic R/ADRA2A Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human alpha-2A Adrenergic R/ADRA2A Antibody

For research use only

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Reviews

Calculators
Flow Cytometry

Human alpha-2A Adrenergic R/ADRA2A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10129

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human alpha-2A Adrenergic R/ADRA2A Antibody

Detection of ADRA2A in HEK293 Human Cell Line Transfected with Human ADRA2A and eGFP by Flow Cytometry.