Human alpha-2B Adrenergic R/ADRA2B Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10324
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 491613
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human alpha‑2B Adrenergic R/ADRA2B
Accession # P18089
Accession # P18089
Specificity
Detects human alpha‑2B Adrenergic R/ADRA2B in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human alpha-2B Adrenergic R/ADRA2B Antibody
Detection of ADRA2B in HEK293 Human Cell Line Transfected with Human ADRA2B and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human ADRA2B or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human ADRA2B Monoclonal Antibody (Catalog # MAB10324) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.Applications for Human alpha-2B Adrenergic R/ADRA2B Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human ADRA2B and eGFP
Sample: HEK293 Human Cell Line Transfected with Human ADRA2B and eGFP
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-2B Adrenergic R/ADRA2B
Alpha-2-adrenergic receptors, including ADRA2B, are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C that have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. ADRA2B was observed to associate with eIF-2B, a guanine nucleotide exchange protein that functions in regulation of translation. A polymorphic variant of ADRA2B was identified to have decreased G protein-coupled receptor kinase-mediated phosphorylation and desensitization; this polymorphic form is also associated with reduced basal metabolic rate in obese subjects and may therefore contribute to the pathogenesis of obesity.
Long Name
Alpha-2B Adrenergic Receptor
Alternate Names
a-2B AdrenergicR, ADRA2B, ADRA2L1, ADRA2RL1, ADRARL1, alpha2B AdrenergicR
Gene Symbol
ADRA2B
UniProt
Additional alpha-2B Adrenergic R/ADRA2B Products
Product Documents for Human alpha-2B Adrenergic R/ADRA2B Antibody
Product Specific Notices for Human alpha-2B Adrenergic R/ADRA2B Antibody
For research use only
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