Human Angiopoietin-like 3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3829
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 370207
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Angiopoietin-like 3
Ser17-Glu460
Accession # Q9Y5C1
Ser17-Glu460
Accession # Q9Y5C1
Specificity
Detects human ANGPTL3 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 40% cross‑reactivity with recombinant mouse (rm) ANGPTL3 and no cross-reactivity with rhAng-1, rhAng-2, or rhAng-4.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human Angiopoietin-like 3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human Angiopoietin-like 3 (Catalog # 3829-AN)
Sample: Recombinant Human Angiopoietin-like 3 (Catalog # 3829-AN)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Angiopoietin-like Protein 3/ANGPTL3
Angiopoietin-like 3 (ANGPTL3) is a secreted glycoprotein that belongs to the angiopoietin family. It is expressed in the liver and circulates as 70 kDa, 50 kDa, and 32 kDa species. These include full length ANGPTL3 as well as the proteolytically separated N- and C-terminal fragments which contain a coiled coil domain and fibrinogen-like domain, respectively. ANGPTL3 promotes an increase in circulating triglyceride levels by directly inhibiting lipoprotein lipase. Mature human ANGPTL3 shares 77% amino acid sequence identity with mouse ANGPTL3.
Alternate Names
AGNPT5, Ang-5, Angiopoietin-5, ANGPTL3
Gene Symbol
ANGPTL3
UniProt
Additional Angiopoietin-like Protein 3/ANGPTL3 Products
- All Products for Angiopoietin-like Protein 3/ANGPTL3
- Angiopoietin-like Protein 3/ANGPTL3 cDNA Clones
- Angiopoietin-like Protein 3/ANGPTL3 ELISA Kits
- Angiopoietin-like Protein 3/ANGPTL3 Lysates
- Angiopoietin-like Protein 3/ANGPTL3 Primary Antibodies
- Angiopoietin-like Protein 3/ANGPTL3 Proteins and Enzymes
- Angiopoietin-like Protein 3/ANGPTL3 Simple Plex
Product Documents for Human Angiopoietin-like 3 Antibody
Product Specific Notices for Human Angiopoietin-like 3 Antibody
For research use only
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