Human Annexin A3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4855
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 693510
Product Specifications
Immunogen
E. coli-derived recombinant human Annexin A3
Ala2-Asp323
Accession # P12429
Ala2-Asp323
Accession # P12429
Specificity
Detects human Annexin A3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5%
cross-reactivity with recombinant human Annexin A1, A2, A4, A6, A8, A9, A10,
A11, or A13 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Annexin A3 Antibody
Detection of Human, Mouse, and Rat Annexin A3 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human Annexin A3 Monoclonal Antibody (Catalog # MAB4855) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Annexin A3 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Annexin A3 in Human Prostate.
Annexin A3 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Annexin A3 Monoclonal Antibody (Catalog # MAB4855) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Annexin A3 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate
Sample: Immersion fixed paraffin-embedded sections of human prostate
Western Blot
0.1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and Rat‑2 rat embryonic fibroblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and Rat‑2 rat embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Annexin A3
Alternate Names
ANXA3, Calcimedin 35-alpha, Lipocortin III, PAP-III
Gene Symbol
ANXA3
UniProt
Additional Annexin A3 Products
Product Documents for Human Annexin A3 Antibody
Product Specific Notices for Human Annexin A3 Antibody
For research use only
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