Human AP-2 gamma Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5059
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human AP-2 gamma
Leu128-Val223
Accession # Q92754
Leu128-Val223
Accession # Q92754
Specificity
Detects human AP-2 gamma in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) AP-2 alpha is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human AP-2 gamma Antibody
AP-2 gamma in Human Breast Cancer Tissue.
AP-2 gamma was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 10 µg/mL Goat Anti-Human AP-2 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5059) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human AP-2 gamma by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line and human thymus tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human AP-2 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5059) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for AP-2 gamma at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human AP-2 gamma Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Western Blot
1 µg/mL
Sample: A431 human epithelial carcinoma cell line and human thymus tissue
Sample: A431 human epithelial carcinoma cell line and human thymus tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: AP-2 gamma
AP-2 gamma (Activation protein 2 gamma; also ERF-1) is a 50-55 kDa member of the AP-2 transcription factor family. It is essential for kidney development and placentation of the embryo. In the nucleus, it forms homodimers and heterodimers with other AP-2 family members. Elevated AP-2 family members are highly suggestive of neoplasia. Human AP-2 gamma is 450 amino acids in length. It contains a repressor SUMOylation site as Lys10, a Gln/Pro-rich transactivation domain (aa 30‑119) and a helix-span-helix dimerization region (aa 293-424). One potential splice form exists that shows a four aa substitution for the N-terminal 16 aa, followed by a premature truncation after Gly130. Over aa 128-223, human AP-2 gamma shares 91% and 82% aa sequence identity with porcine and mouse AP-2 gamma, respectively.
Long Name
Activating Enhancer-binding Protein gamma
Alternate Names
AP2 gamma, ERF1, TFAP2C, TFAP2G
Gene Symbol
TFAP2C
UniProt
Additional AP-2 gamma Products
Product Documents for Human AP-2 gamma Antibody
Product Specific Notices for Human AP-2 gamma Antibody
For research use only
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