U-118-MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human APJ Monoclonal Antibody (Catalog # MAB8561, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

APJ in Human Hypothalamus.

APJ was detected in immersion fixed paraffin-embedded sections of human hypothalamus using Mouse Anti-Human APJ Monoclonal Antibody (Catalog # MAB8561) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human APJ Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: U‑118‑MG human glioblastoma/astrocytoma cell line

Immunohistochemistry

0.5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human hypothalamus

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APJ

APJ is a G-protein coupled receptor that is related to the angiotensin receptor. It is primarily expressed in coronary artery endothelium and binds the endogenous ligand, apelin. APJ can also function as a co-receptor for HIV.

Long Name

Apelin Receptor/G-protein Coupled Receptor APJ

Alternate Names

AGTRL1, APJR, APLNR, HG11

Entrez Gene IDs

187 (Human); 23796 (Mouse); 83518 (Rat)

Gene Symbol

APLNR

UniProt

P35414

Additional APJ Products

Product Documents for Human APJ Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human APJ Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human APJ Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8561

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all APJ Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human APJ Antibody

Detection of APJ in U‑118‑MG Human Cell Line by Flow Cytometry.