Human Apolipoprotein C-II/ApoC2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4497
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human ApoC2
Thr23-Glu101
Accession # P02655
Thr23-Glu101
Accession # P02655
Specificity
Detects human ApoC2 in Western blots. In Western blots, this antibody detects both the precursor and processed forms of ApoC2.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Apolipoprotein C-II/ApoC2 Antibody
Detection of Human Apolipoprotein C-II/ApoC2 by Western Blot.
Western blot shows lysates of human small intestine tissue and plasma. PVDF membrane was probed with 0.5 µg/mL of Human Apolipoprotein C-II/ApoC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4497) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for precursor Apolipoprotein C-II/ApoC2 at approximately 12 kDa and processed ApoC2 at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human Apolipoprotein C‑II/ApoC2 by Simple WesternTM.
Simple Western lane view shows lysates of human plasma, loaded at 0.2 mg/mL. A specific band was detected for Apolipoprotein C-II/ApoC2 at approximately 9 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human Apolipoprotein C-II/ApoC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4497) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Apolipoprotein C-II/ApoC2 Antibody
Application
Recommended Usage
Simple Western
5 µg/mL
Sample: Human plasma
Sample: Human plasma
Western Blot
0.5 µg/mL
Sample: Human small intestine tissue and plasma
Sample: Human small intestine tissue and plasma
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Apolipoprotein C-II/ApoC2
Apolipoprotein C2 (ApoC2/II) is a 8‑9 kDa, secreted member of the Apolipoprotein C2 family of proteins. It is produced by hepatocytes and represents a major component of VLDL particles. It activates lipoprotein lipase and may self-associate to form amyloid-type fibrils. The human ApoC2 precursor is 101 amino acids (aa) in length. It contains a 22 amino acid (aa) signal sequence, followed by a 79 aa ProApoC2 that contains a lipid-binding region (aa 43‑51) and an enzyme interaction site (aa 55‑78). ProApoC2 represents >90% of circulating ApoC2. In human, limited proteolytic processing occurs with removal of the six aa prosegment (aa 23‑28). This does not affect activity. Human ProApoC2 is 59% and 62% aa identical to mouse and rat ProApoC2, respectively.
Alternate Names
APOC2, Apolipoprotein CII
Gene Symbol
APOC2
UniProt
Additional Apolipoprotein C-II/ApoC2 Products
Product Documents for Human Apolipoprotein C-II/ApoC2 Antibody
Product Specific Notices for Human Apolipoprotein C-II/ApoC2 Antibody
For research use only
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