Human Apolipoprotein C-II/ApoC2 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF4497
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human ApoC2
Thr23-Glu101
Accession # P02655
Thr23-Glu101
Accession # P02655
Specificity
Detects recombinant and endogenous human ApoC2 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Apolipoprotein C-II/ApoC2 Biotinylated Antibody
Detection of Human Apolipoprotein C‑II/ApoC2 by Western Blot.
Western blot shows lysates of human plasma and small intestine tissue. PVDF membrane was probed with 0.25 µg/mL of Human Apolipoprotein C-II/ApoC2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4497) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein C-II/ApoC2 at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human Apolipoprotein C-II/ApoC2 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.25 µg/mL
Sample: Human plasma and small intestine tissue
Sample: Human plasma and small intestine tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Apolipoprotein C-II/ApoC2
Apolipoprotein C2 (ApoC2/II) is a 8‑9 kDa, secreted member of the Apolipoprotein C2 family of proteins. It is produced by hepatocytes and represents a major component of VLDL particles. It activates lipoprotein lipase and may self-associate to form amyloid-type fibrils. The human ApoC2 precursor is 101 amino acids (aa) in length. It contains a 22 amino acid (aa) signal sequence, followed by a 79 aa ProApoC2 that contains a lipid-binding region (aa 43‑51) and an enzyme interaction site (aa 55‑78). ProApoC2 represents > 90% of circulating ApoC2. In human, limited proteolytic processing occurs with removal of the six aa prosegment (aa 23‑28). This does not affect activity. Human ProApoC2 is 59% aa identical to mouse and dog ProApoC2, respectively.
Alternate Names
APOC2, Apolipoprotein CII
Gene Symbol
APOC2
UniProt
Additional Apolipoprotein C-II/ApoC2 Products
Product Documents for Human Apolipoprotein C-II/ApoC2 Biotinylated Antibody
Product Specific Notices for Human Apolipoprotein C-II/ApoC2 Biotinylated Antibody
For research use only
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