Human APP/Protease Nexin II (Amyloid beta) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB96182
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG Clone # AB42-5
Product Specifications
Immunogen
Recombinant protein to Fibrillar Amyloid beta 1-42 peptide
Specificity
Detects Human APP/Protease Nexin II (amino acids 1-11 of Amyloid beta) in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG
Scientific Data Images for Human APP/Protease Nexin II (Amyloid beta) Antibody
Human Amyloid beta (aa1-40) ELISA Standard Curve.
Recombinant Human Amyloid beta protein was serially diluted 2-fold and captured by Mouse Anti-Human Amyloid beta (aa1-40) Monoclonal Antibody (Catalog # MAB96181or Catalog # MAB96181R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human APP/Protease Nexin II (Amyloid beta) Monoclonal Antibody (Catalog # MAB96182) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Human Amyloid beta (aa1-42) ELISA Standard Curve.
Recombinant Human Amyloid beta protein was serially diluted 2-fold and captured by Mouse Anti-Human Amyloid beta (aa1-42) Monoclonal Antibody (Catalog # MAB9618or Catalog # MAB9618R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human APP/Protease Nexin II (Amyloid beta) Monoclonal Antibody (Catalog # MAB96182) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human APP/Protease Nexin II (Amyloid beta) Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Amyloid beta (aa1-40) Monoclonal Antibody (Catalog # MAB96181 or Catalog # MAB96181R) or Mouse Anti-Human Amyloid beta (aa1-42) Monoclonal Antibody (Catalog # MAB9618 or Catalog # MAB9618R).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Amyloid beta (aa1-40) Quantikine ELISA Kit (Catalog # DAB140B) or Human Amyloid beta (aa1-42) Quantikine ELISA Kit (Catalog # DAB142) for a complete optimized ELISA.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: APP
Long Name
Amyloid Precursor Protein
Alternate Names
Amyloid beta, beta Amyloid, Protease Nexin II
Gene Symbol
APP
Additional APP Products
Product Documents for Human APP/Protease Nexin II (Amyloid beta) Antibody
Product Specific Notices for Human APP/Protease Nexin II (Amyloid beta) Antibody
For research use only
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