Human APRIL/TNFSF13 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC884P

R&D Systems, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Polyclonal Goat IgG

View all APRIL/TNFSF13 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human APRIL/TNFSF13

Specificity

Detects human APRIL/TNFSF13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Fas Ligand, rhTNF-alpha, rhTRAIL, and rhTRANCE is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human APRIL/TNFSF13 PE-conjugated Antibody

Detection of APRIL/TNFSF13 antibody in Th2-stimulated PBMCs antibody by Flow Cytometry.

Detection of APRIL/TNFSF13 in Th2-stimulated PBMCs by Flow Cytometry.

Th2-stimulated peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human APRIL/TNFSF13 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC884P) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (FAB3791A). Quadrant markers were set based on control antibody staining (IC108P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of APRIL/TNFSF13 in U937 Human Cell Line by Flow Cytometry.

U937 human lymphoma cell line was stained with Goat Anti-Human APRIL/TNFSF13 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC884P, filled histogram) or control antibody staining (IC108P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human APRIL/TNFSF13 PE-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: U937 human lymphoma cell line and Th2-stimulated peripheral blood mononuclear cells (PBMCs) fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: APRIL/TNFSF13

APRIL (A Proliferation-inducing Ligand), also known as TNFSF13, TALL2, and TRDL1, is a member of the TNF ligand superfamily. APRIL is synthesized as a 32 kDa type II transmembrane protein which is cleaved by furin in the Golgi to release a 17 kDa soluble molecule. Secreted APRIL consists almost entirely of a single TNF homology domain. Little or no transmembrane APRIL is expressed on the cell surface. Alternate splicing generates isoforms with short deletions at the N- or C-terminus. Human APRIL shares 85% aa sequence identity with mouse and rat APRIL. Among TNF superfamily ligands, BAFF shows the greatest sequence homology with APRIL, and the two proteins exhibit overlapping biological activities. APRIL promotes cellular proliferation and provides protection from apoptosis in normal and transformed cells. It is present in elevated amounts in a wide variety of cancers, primarily due to expression by tumor-infiltrating neutrophils, and is known to be expressed by T cells, macrophages, B cells, adipocytes and capillary endothelial cells. Both APRIL and BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, and BAFF additionally functions through BAFF R. A stretch of basic amino acids at the N-terminus of APRIL is required for its interaction with Heparan Sulfate Proteoglycans (HSPGs). Binding to HSPGs is independent of APRIL’s binding to TACI and BCMA. Interaction with HSPGs serves to concentrate APRIL on the surface of cells, thereby favoring TACI- or BCMA-mediated effects. APRIL can form bioactive heterotrimers with BAFF, and these circulate in the serum of patients with rheumatic immune disorders. A bioactive protein known as TWE-PRIL consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL. TWE-PRIL is expressed in monocytes and activated T cells, and in contrast to APRIL, is presented on the cell surface.

Long Name

A Proliferation-inducing Ligand

Alternate Names

CD256, TALL2, TNFSF13, TRDL1, ZTNF2

Entrez Gene IDs

8741 (Human); 69583 (Mouse); 287437 (Rat)

Gene Symbol

TNFSF13

Additional APRIL/TNFSF13 Products

Product Documents for Human APRIL/TNFSF13 PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human APRIL/TNFSF13 PE-conjugated Antibody

For research use only

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