Western blot shows lysates of human liver tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human ASGR2 Monoclonal Antibody (Catalog # MAB9970) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ASGR2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of ASGR2 in HEK293 Human Cell Line Transfected with Human ASGR2 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) human ASGR2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human ASGR2 Monoclonal Antibody (Catalog # MAB9970) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

ASGR2 in Human Liver.

ASGR2 was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human ASGR2 Monoclonal Antibody (Catalog # MAB9970) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human ASGR2 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human ASGR2 and eGFP

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Western Blot

2 µg/mL
Sample: Human liver tissue

Reviewed Applications

Read 1 review rated 5 using MAB9970 in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.

Background: ASGR2

Asialoglycoprotein receptor 2 (ASGR2) is a subunit of the Asialoglycoprotein receptor. The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by different genes. The protein encoded by this gene is the less abundant minor subunit. The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. Expressed on hepatocytes, the Asialoglycoprotein receptor mediates endocytosis and lysosomal degradation of glycoproteins to mediate serum glycoprotein homeostasis. The ASGR receptor binds plasma glycoproteins which have had the terminal sialic acid residue removed. An alternatively spliced variant, H2, which can be shed, has been proposed as a marker for liver fibrosis.

Long Name

Asialoglycoprotein Receptor 2

Alternate Names

ASGPR2, CLEC4H2, HBXBP

Entrez Gene IDs

433 (Human); 11890 (Mouse); 29403 (Rat)

Gene Symbol

ASGR2

UniProt

P07307

Additional ASGR2 Products

Product Documents for Human ASGR2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ASGR2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human ASGR2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9970

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all ASGR2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human ASGR2 Antibody

Detection of Human ASGR2 by Western Blot.