Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Western Blot

Cited:

ELISA Development, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 137314

View all BAFF/BLyS/TNFSF13B Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human BAFF/BLyS/TNFSF13B
Ala81-Leu285
Accession # Q9Y275

Specificity

Detects human BAFF/BLyS/TNFSF13B in direct ELISAs and Western blots. In Western blots, approximately 5% cross‑reactivity with recombinant human (rh) TL1A/TNSF15 is observed and no cross-reactivity with rhAPRIL, rhTNF-alpha, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhTRAIL, rhTRANCE, or rhTWEAK is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human BAFF/BLyS/TNFSF13B Antibody

Detection of BAFF/BLyS/TNFSF13B in THP-1 cells by Flow Cytometry

THP-1 cells were stained with Mouse Anti-Human BAFF/BLyS/TNFSF13B Monoclonal Antibody (Catalog # MAB1241, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human BAFF/BLyS/TNFSF13B Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: THP-1 cells 

Western Blot

1 µg/mL
Sample: Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB1241 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAFF/BLyS/TNFSF13B

BAFF (also known as TALL-1, BLyS, and THANK) is a type II transmembrane glycoprotein belonging to the TNF superfamily and has been designated as TNF superfamily member 13B (TNFSF13B). Human BAFF is a 285 amino acid (aa) protein consisting of a 218 aa extracellular domain, a 21 aa transmembrane region and a 46 aa cytoplasmic tail (1, 2). BAFF has the typical structural characteristics of the TNF superfamily ligands. It is a homotrimeric protein having the structurally conserved motif known as TNF homology domain (3, 4). A higher ordered structure composed of a cluster of trimeric units resembling the structure of a viral capsid has also been reported (4). Human BAFF may be shed from the cell surface by proteolytic cleavage between R133 and Ala134 to yield a soluble form of the protein that is detectable in serum (1, 5). Within the TNF superfamily BAFF shares the highest homology (48%) with APRIL (1). BAFF shares with APRIL the ability to bind to BCMA and TACI and also binds specifically to BAFF receptor (BAFF R, also known as BR3 or TNFSFR13C), which is the principal BAFF receptor (6 - 8). All three receptors are type III transmembrane proteins that are expressd in B cells. BAFF and APRIL can form active heteromers that bind to TACI (9). BAFF is expressed in peripheral blood mononuclear cells, in spleen and lymph nodes. Its expression in resting monocytes is up-regulated by IFN-alpha, IFN-beta, LPS and IL-10. BAFF provides critical survival signals to a subset of B cells with intermediate maturation status (T2 B cells) during the immune response (10). BAFF also plays an important role in the development of lymphoid tissue and enhances the survival of activated memory B cells (7, 11). Human and mouse BAFF share 86% aa sequence identity (1).

References

  1. Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
  2. Mukhopadhyay, A. et al. (1999) J. Biol. Chem. 274:15978.
  3. Karpusas, M. et al. (2002) J. Mol. Biol. 315:1145.
  4. Liu, Y. et al. (2002) Cell 108:383.
  5. Cheema, G.S. et al. (2001) Arthr. Rheum. 44:1313.
  6. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
  7. Thompson, J.S. et al. (2001) Science 293:2108.
  8. Ng, L.G. et al. (2004) J. Immunol. 173:807.
  9. Roschke, V. et al. (2002) J. Immunol. 169:4314.
  10. Batten, M. et al. (2000) J. Exp. Med. 192:1453.
  11. Avery, D.T. et al. (2003) J. Clin. Invest. 112:286.

Long Name

B cell Activating Factor

Alternate Names

BLyS, CD257, TALL1, THANK, TNFSF13B, ZTNF4

Entrez Gene IDs

10673 (Human); 24099 (Mouse); 498666 (Rat); 102123214 (Cynomolgus Monkey)

Gene Symbol

TNFSF13B

UniProt

Q9Y275

Additional BAFF/BLyS/TNFSF13B Products

Product Documents for Human BAFF/BLyS/TNFSF13B Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human BAFF/BLyS/TNFSF13B Antibody

For research use only

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