Human BAP31 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5574
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human BAP31
Gly165-Glu246
Accession # P51572
Gly165-Glu246
Accession # P51572
Specificity
Detects human BAP31 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human BAP31 Antibody
Detection of Human BAP31 by Western Blot.
Western blot shows lysates of Nalm-6 human Pre-B acute lymphocytic leukemia cell line, human heart tissue and human cortex tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human BAP31 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5574) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for BAP31 at approximately 31 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human BAP31 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Nalm-6 human Pre-B acute lymphocytic leukemia cell line, human heart tissue and human cortex tissue
Sample: Nalm-6 human Pre-B acute lymphocytic leukemia cell line, human heart tissue and human cortex tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BAP31
BAP31 (BCR-associated protein of 31 kDa) is a 29 kDa member of the BCAP29/BCAP31 family of proteins. It is ubiquitously expressed, resides in the ER and serves multiple functions. It is known to act as a cargo receptor for ER export of transmembrane (TM) proteins, and serve as a component of the ER quality control compartment, cycling between the peripheral and juxtanuclear ER. Human BAP31 is a 246 amino acid (aa), 3-TM domain containing protein. Amino acids 1‑6 are lumenal, 124‑246 are cytoplasmic, and 7‑27, 44‑64 and 103‑123 constitute TM segments. BAP31 forms homodimers and heterodimers with BAP29. Cleavage between Asp164Gly165 and Asp238Gly239 generates 20 kDa and 27 kDa fragments, respectively. There is an alternate start site 67 aa upstream from the standard start site. Over aa 165‑246, human BAP31 shares 82% aa identity with mouse BAP31.
Long Name
B-cell Receptor-associated Protein 31
Alternate Names
6C6-Ag, BCAP31
Gene Symbol
BCAP31
UniProt
Additional BAP31 Products
Product Documents for Human BAP31 Antibody
Product Specific Notices for Human BAP31 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...