Human Bcl-10 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2889
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Bcl-10
Met1-Leu192
Accession # O95999
Met1-Leu192
Accession # O95999
Specificity
Detects human Bcl-10 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Bcl-10 Antibody
Detection of Human Bcl-10 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A431 human epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Bcl-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2889) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Bcl-10 at approximately 28 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 4.
Bcl‑10 in A431 Human Cell Line.
Bcl-10 was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human Bcl-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2889) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Bcl-10 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line
Sample: Immersion fixed A431 human epithelial carcinoma cell line
Western Blot
0.5 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, A431 human epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line
Sample: Raji human Burkitt's lymphoma cell line, A431 human epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bcl-10
Long Name
B Cell Lymphoma/Leukemia 10
Alternate Names
Bcl10, c-E10, CARMEN, CIPER, CLAP, mE10
Entrez Gene IDs
8915 (Human)
Gene Symbol
BCL10
UniProt
Additional Bcl-10 Products
Product Documents for Human Bcl-10 Antibody
Product Specific Notices for Human Bcl-10 Antibody
For research use only
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