Skip to main content

Human Bcl-w Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB824

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
MAB824
MAB824-SP

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 90316

Product Specifications

Immunogen

E. coli-derived recombinant human Bcl-w
Met1-Thr172
Accession # Q92843

Specificity

Detects human Bcl-w in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Bcl-w Antibody

Detection of Human Bcl-w antibody by Western Blot.

Detection of Human Bcl-w by Western Blot.

Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and CTLL-2 mouse cytotoxic T cell line. The PVDF membrane was probed with 1 µg/mL Human Bcl-w Monoclonal Antibody (Catalog # MAB824) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human Bcl-w Minus C-Terminus (Catalog # 824-BW) (1 ng) was included. A specific band for Bcl-w was detected at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9with 25 mM Tris and 0.15 M NaCl.

Applications for Human Bcl-w Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: WS-1 human fetal skin fibroblast cell line and CTLL-2 mouse cytotoxic T cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

For liquid material, refer to CoA for concentration.

Formulation

Supplied as a 0.2 μm filtered solution in PBS with Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: Bcl-w

Bcl-w is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-w is required for normal sperm maturation (3, 4, 5). Natural Bcl-w contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-w missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-w appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.

 

References

  1. Gross, A. et al. (1999) Genes and Develop. 13:1899.
  2. Kroemer, G. (1997) Nature Med. 3:614.
  3. Ross, J.A., et al. (1998) Nat. Genet. 18:251.
  4. Print, C.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:12323.
  5. Yan, W. et al. (2000) Mol. Endocrin. 14:682.

Long Name

B Cell Lymphoma/Leukemia w

Alternate Names

BCL2L2, Bclw

Entrez Gene IDs

599 (Human); 12050 (Mouse)

Gene Symbol

BCL2L2

UniProt

Additional Bcl-w Products

Product Documents for Human Bcl-w Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Bcl-w Antibody

For research use only

Loading...
Loading...
Loading...
Loading...