RPMI8226 human myeloma cell line was stained with Mouse Anti-Human BCMA/TNFRSF17 Monoclonal Antibody (Catalog # MAB1932, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human BCMA/TNFRSF17 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human BCMA/TNFRSF17 Antigen Affinity-purified Polyclonal Antibody(Catalog # AF193).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human BCMA/TNFRSF17 DuoSet ELISA Kit (Catalog # DY193) for convenient development of a sandwich ELISA.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: RPMI8226 human myeloma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB1932 in the following applications:

ELISA (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BCMA/TNFRSF17

BCMA, B cell maturation antigen, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.

References

Madry, C. et al. (1998) Int. Immunol. 10:1693.
Gras, M. et al. (1995) Int. Immunol. 7:1093.
Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
Marsters, S. et al. (2000) Curr. Biol. 10:785.
Thompson, J. et al. (2000) J. Exp. Med. 192:129.

Long Name

B Cell Maturation Factor

Alternate Names

CD269, TNFRSF13A, TNFRSF17

Entrez Gene IDs

608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)

Gene Symbol

TNFRSF17

UniProt

Q02223

Additional BCMA/TNFRSF17 Products

Product Documents for Human BCMA/TNFRSF17 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human BCMA/TNFRSF17 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human BCMA/TNFRSF17 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1932

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all BCMA/TNFRSF17 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human BCMA/TNFRSF17 Antibody

Detection of BCMA/TNFRSF17 in RPMI8226 Human Cell Line by Flow Cytometry.