Human BCMA/TNFRSF17 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1933
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Blockade of Receptor-ligand Interaction, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1039701
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BCMA /TNFRSF17
Met1-Ala54
Accession # Q02223
Met1-Ala54
Accession # Q02223
Specificity
Detects human BCMA/TNFRSF17 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human BCMA/TNFRSF17 Antibody
Detection of BCMA/TNFRSF17 in RPMI8226 Human Cell Line by Flow Cytometry.
RPMI8226 human myeloma cell line was stained with Mouse Anti-Human BCMA/TNFRSF17 Monoclonal Antibody (Catalog # MAB1933, filled histogram) or Mouse IgG1 isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol.
BCMA/TNFRSF17 Fc Chimera binding to APRIL/TNFSF13 is Blocked by BCMA/TNFRSF17 Antibody
Human BCMA/TNFRSF17 Antibody (Catalog # MAB1933) blocks the binding of Recombinant Human BCMA/TNFRSF17 Fc Chimera (193-BC) to Recombinant Human APRIL/TNFSF13 (5860-AP) in a functional ELISA binding assay. The ND50 for this effect is 0.500-6.00 µg/mL.Applications for Human BCMA/TNFRSF17 Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
Flow Cytometry
0.25 µg/106 cells
Sample: RPMI8226 human myeloma cell line
Sample: RPMI8226 human myeloma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BCMA/TNFRSF17
BCMA, B cell maturation antigen, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.
References
- Madry, C. et al. (1998) Int. Immunol. 10:1693.
- Gras, M. et al. (1995) Int. Immunol. 7:1093.
- Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
- Marsters, S. et al. (2000) Curr. Biol. 10:785.
- Thompson, J. et al. (2000) J. Exp. Med. 192:129.
Long Name
B Cell Maturation Factor
Alternate Names
CD269, TNFRSF13A, TNFRSF17
Gene Symbol
TNFRSF17
UniProt
Additional BCMA/TNFRSF17 Products
Product Documents for Human BCMA/TNFRSF17 Antibody
Product Specific Notices for Human BCMA/TNFRSF17 Antibody
For research use only
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