Human beta-Synuclein Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5528
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 516505
Product Summary for Human beta-Synuclein Antibody
Immunogen
E. coli-derived recombinant human beta-Synuclein
Gly47-Glu120
Accession # Q16143
Gly47-Glu120
Accession # Q16143
Specificity
Detects human beta-Synuclein in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human alpha-Synuclein is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human beta-Synuclein Antibody
Detection of Human beta-Synuclein by Western Blot.
Western blot shows lysates of human hippocampal tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human beta-Synuclein Monoclonal Antibody (Catalog # MAB5528) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for beta-Synuclein at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of beta-Synuclein in Human Hippocampus.
beta-Synuclein was detected in immersion fixed paraffin-embedded sections of Human Hippocampus using Mouse Anti-Human beta-Synuclein Monoclonal Antibody (Catalog # MAB5528) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human beta-Synuclein Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Hippocampus
Sample: Immersion fixed paraffin-embedded sections of Human Hippocampus
Western Blot
2 µg/mL
Sample: Human hippocampal tissue
Sample: Human hippocampal tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB5528 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-Synuclein
Alternate Names
SNCB, Synuclein-beta
Gene Symbol
SNCB
UniProt
Additional beta-Synuclein Products
Product Documents for Human beta-Synuclein Antibody
Product Specific Notices for Human beta-Synuclein Antibody
For research use only
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