Human BMP-15/GDF-9B Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2925
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Gln268-Arg392
Accession # O95972
Specificity
Clonality
Host
Isotype
Applications for Human BMP-15/GDF-9B Antibody
Western Blot
Sample: Recombinant Human BMP‑15/GDF‑9B
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-15/GDF-9B
Bone morphogenetic protein 15 (BMP-15), also known as GDF-9B, is a TGF-beta superfamily ligand that is expressed by oocytes throughout folliculogenesis, and plays an important role in oocyte development (1). BMP-15 promotes the FSH-independent proliferation of ovarian granulosa cells (GC) and induces GC glycolysis and cholesterol synthesis (2‑4). It also induces GC production of stem cell factor which, in turn, negatively regulates BMP-15 expression in oocytes (5). BMP-15 blocks the FSH-induced GC expression of FSH R and multiple steroidogenic molecules (6). BMP-15 is synthesized with a 249 amino acid (aa) N-terminal propeptide (7). The propeptide is cleaved intracellularly from the 50 kDa proBMP-15 but remains associated with mature BMP-15 (8). Mature BMP-15 exists in 16 kDa and 17 kDa forms which are distinguishable by the presence of O-linked glycosylation on the 17 kDa form (8). Mature BMP-15 is phosphorylated, a modification which is required for the stimulation of GC proliferation (9). BMP-15 exerts its effects through interactions with BMPR-IB/ALK6 and BMPR-II (9‑11). Mature BMP-15 forms 34 kDa noncovalently-linked homodimers and 37 kDa heterodimers with mature GDF-9 (12). Both of these oocyte-expressed factors lack the cysteine that mediates disulfide-linked dimerization in most TGF-beta superfamily ligands (1). Although heterodimerization with GDF-9 may limit the secretion of active BMP-15, these two factors synergize in promoting oocyte survival and folliculogenesis (12, 13). Mature human BMP-15 shares 70%, 68%, and 78% aa sequence identity with mouse, rat, and sheep BMP-15, respectively. It shares 27%‑38% aa sequence with other BMPs.
References
- Moore, R.K. and S. Shimasaki (2005) Mol. Cell. Endocrinol. 234:67.
- Otsuka, F. et al. (2000) J. Biol. Chem. 275:39523.
- Sugiura, K. et al. (2007) Development 134:2593.
- Su, Y.-Q. et al. (2008) Development 135:111.
- Otsuka, F. and S. Shimasaki (2002) Proc. Natl. Acad. Sci. 99:8060.
- Otsuka, F. et al. (2001) J. Biol. Chem. 276:11387.
- Dube, J.L. et al. (1998) Mol. Endocrinol. 12:1809.
- Saito, S. et al. (2008) Prot. Sci. 17:362.
- McMahon, H.E. et al. (2008) Endocrinology 149:812.
- Moore, R.K. et al. (2003) J. Biol. Chem. 278:304.
- Edwards, S.J. et al. (2008) Endocrinology 149:1026.
- Liao, W.X. et al. (2003) J. Biol. Chem. 278:3713.
- Yan, C. et al. (2001) Mol. Endocrinol. 15:854.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional BMP-15/GDF-9B Products
Product Documents for Human BMP-15/GDF-9B Antibody
Product Specific Notices for Human BMP-15/GDF-9B Antibody
This product is sold under license from Vanderbilt University for research and laboratory use only. Licenses for commercial manufacture, commercial use, diagnostics or therapeutics may beobtained by contacting Vanderbilt University, Nashville, Tennessee.
For research use only