Human BMP-4 MAb (Clone 66119)

R&D Systems, part of Bio-Techne | Catalog # MAB757

R&D Systems, part of Bio-Techne
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MAB757-100
MAB757-500
MAB757-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Ovine

Applications

Validated:

Neutralization, Western Blot

Cited:

Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 66119

View all BMP-4 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BMP-4
Ser293-Arg408
Accession # P12644

Specificity

Detects human BMP-4 in direct ELISAs and Western blots. This antibody shows approximately 5% cross-reactivity with recombinant human BMP‑2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human BMP-4 MAb (Clone 66119)

Alkaline Phosphatase Production Induced by BMP-4 and Neutralization by Human BMP-4 Antibody.

Alkaline Phosphatase Production Induced by BMP-4 and Neutralization by Human BMP-4 Antibody.

Recombinant Human BMP-4 (Catalog # 314-BP) induces alkaline phosphatase production in the the C2C12 mouse myoblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-4 (500 ng/mL) is neutralized (green line) by increasing concentrations of Human BMP-4 Monoclonal Antibody (Catalog # MAB757). The ND50 is typically 1-3 µg/mL in the presence of heparin (2 µg/mL).

Applications for Human BMP-4 MAb (Clone 66119)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human BMP-4 (Catalog # 314-BP)

Neutralization

Measured by its ability to neutralize BMP‑4-induced alkaline phosphatase production in the C2C12 mouse myoblast cell line. Katagiri, T. et al. (1994) J. Cell Biol. 127:1755. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 500 ng/mL Recombinant Human BMP‑4 and 2 µg/mL heparin.

Reviewed Applications

Read 5 reviews rated 4.2 using MAB757 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-4

BMP4 is a TGF beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1 5). The human BMP4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfidelinked homodimeric BMP4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7 disulfidelinked heterodimer (7 9). Mature human and mouse BMP4 share 98% aa sequence identity. Human BMP4 shares 85% aa sequence identity with human BMP2 and less than 50% with other human BMPs. Compared to BMP4 homodimers, BMP4/ BMP7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPRIA) and type II (primarily Activin RIIA or BMPRII) receptors (11, 12). The bioavailability of BMP4 is regulated by its interaction with multiple proteins and glycosaminoglycan (13 - 15).

References

  1. Zhang, P. et al., 2008, Blood 111:1933.
  2. Gambaro, K. et al., 2006, Cell Death Differ. 13:1075.
  3. Simic, P.  and Vukicevic, S., 2005, Cytokine Growth Factor Rev. 16:299.
  4. Sadlon, T.J. et al., 2004, Stem Cells 22:457.
  5. Frank, D.B. et al., 2005, Circ. Res. 97:496.
  6. Wozney, J. et al., 1988, Science 242:1528.
  7. Cui, Y. et al., 1998, EMBO J. 17:4735.
  8. Cui, Y. et al., 2001, Genes Dev. 15:2797.
  9. Aono, A. et al., 1995, Biochem. Biophys. Res. Commun. 210:670.
  10. Nishimatsu, S. and G.H. Thomsen, 1998, Mech. Dev. 74:75.
  11. Chen, D. et al., 2004, Growth Factors 22:233.
  12. Lavery, K. et al., 2008, J. Biol. Chem. 283:20948.
  13. Rosen, V., 2006, Ann. N.Y. Acad. Sci. 1068:19.
  14. Jones, C.M. and J.C. Smith, 1998, Dev. Biol. 194:12.
  15. Takada, T. et al., 2003, J. Biol. Chem. 278:43229.

Long Name

Bone Morphogenetic Protein 4

Alternate Names

BMP2B, BMP2B1, BMP4, MCOPS6, OFC11

Entrez Gene IDs

652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)

Gene Symbol

BMP4

UniProt

P12644

Additional BMP-4 Products

Product Documents for Human BMP-4 MAb (Clone 66119)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human BMP-4 MAb (Clone 66119)

For research use only

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