Human BNIP3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB41471

R&D Systems, part of Bio-Techne
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MAB41471-100
MAB41471-SP
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 670639

View all BNIP3 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human BNIP3
Gly2-Ser160
Accession # Q12983

Specificity

Detects human BNIP3 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human BNIP3 Antibody

Detection of Human BNIP3 antibody by Western Blot.

Detection of Human BNIP3 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with CoCl2.PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human BNIP3 Polyclonal Antibody (Catalog # MAB41471) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for BNIP3 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
BNIP3 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

BNIP3 in HeLa Human Cell Line.

BNIP3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human BNIP3 Polyclonal Antibody (Catalog # MAB41471) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human BNIP3 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Western Blot

0.5 µg/mL
Sample: MCF‑7 human breast cancer cell line treated with CoCl2

Reviewed Applications

Read 1 review rated 5 using MAB41471 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BNIP3

Bcl-2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3), also known as 19 kDa interacting protein 3 (NIP3), is a proapoptotic member of Bcl-2 protein family. BNIP3 is a 194 amino acid, 21.5 kDa (predicted) protein that contains a single Bcl-2 homology 3 (BH3) domain and a C-terminal transmembrane domain required for mitochondrial localization, homodimerization, and regulation of its proapoptotic function. BNIP3 was identified as one of several proteins that interact with discrete domains of Bcl-2 and the E1B 19 kDa protein. Under conditions of prolonged oxygen deprivation, the hypoxia-induced protein HIF1-alpha activates expression of BNIP3, which in turn, promotes apoptosis under these conditions. The mechanism of BNIP3-mediated apoptosis is independent of caspase activation and cytochrome c release and is characterized by early plasma membrane and mitochondrial damage, prior to the appearance of chromatin condensation or DNA fragmentation. Human BNIP3 shares 90% amino acid identity with mouse and rat BNIP3. Human BNIP3 shares 56% amino acid sequence identity with human BNIP3L.

Long Name

BCL2/Adenovirus E1B 19 Kda Protein-Interacting Protein 3-Like

Alternate Names

Nip3

Entrez Gene IDs

664 (Human); 12176 (Mouse); 84480 (Rat)

Gene Symbol

BNIP3

UniProt

Q12983

Additional BNIP3 Products

Product Documents for Human BNIP3 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human BNIP3 Antibody

For research use only

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