Human BVES Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4986
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human BVES
Met1-Asn36
Accession # Q8NE79
Met1-Asn36
Accession # Q8NE79
Specificity
Detects human BVES in direct ELISAs
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human BVES Antibody
Detection of BVES in Human Heart.
BVES was detected in immersion fixed paraffin-embedded sections of Human Heart using Sheep Anti-Human BVES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4986) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human BVES Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Heart
Sample: Immersion fixed paraffin-embedded sections of Human Heart
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BVES
BVES (blood vessel epicardial substance; also Popeye 1) is a 58 kDa, glycoprotein member of the Popeye family of multispan transmembrane (TM) molecules. It is expressed on embryonic epicardial epithelium plus atrial myocytes and on adult skeletal and cardiac muscle. It serves as a component of the epithelial tight junction, dimerizing with itself and interacting with ZO-1. Human BVES is 360 amino acids (aa) in length. It contains an extracellular N-terminus of 48 aa, three TM domains (aa 49‑113) and a large C-terminal cytoplasmic region (aa 114‑360) that contains a Popeye domain (aa 123‑266). Homodimerization occurs via aa 268‑274. There is one potential splice variant that shows a premature truncation after Glu213. Over aa 1‑36, human BVES is 72% aa identical to mouse BVES.
Long Name
Blood Vessel Epicardial Substance
Alternate Names
POP1, POPDC1
Gene Symbol
BVES
UniProt
Additional BVES Products
Product Documents for Human BVES Antibody
Product Specific Notices for Human BVES Antibody
For research use only
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