Human c-Myc PerCP-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC3696C
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation = 482 and 564 nm, Emission = 675 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 9E10
Product Specifications
Immunogen
C-terminal region peptide of human c-Myc
Ala408-Ala439
Accession # P01106
Ala408-Ala439
Accession # P01106
Specificity
Detects endogenous human c-Myc and c-Myc tagged proteins in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human c-Myc PerCP-conjugated Antibody
Detection of c‑Myc in Jurkat Human Cell Line by Flow Cytometry.
Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human c-Myc PerCP-conjugated Monoclonal Antibody (Catalog # IC3696C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.Applications for Human c-Myc PerCP-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Jurkat human acute T cell leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol
Sample: Jurkat human acute T cell leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: c-Myc
Human c-Myc is a helix-loop-helix transcription factor which efficiently binds DNA after heterodimerization with the bHLH protein Max. It is often overexpressed and mutated in hematopoietic tumors. Mutations frequently result in truncation around amino acid (aa) 252, before the C-terminal DNA binding, HLH and leucine zipper domains. The 439 aa human c-Myc has one O-glycosylation site and has three Ser/Thr phosphorylation sites near the N-terminus. Human c-Myc shows 92% aa identity with mouse or rat c-Myc.
Long Name
v-Myc Avian Myelocytomatosis Viral Oncogene Homolog (Avian)
Alternate Names
cMyc, Myc, Myc2, Niard, Nird
Gene Symbol
MYC
UniProt
Additional c-Myc Products
Product Documents for Human c-Myc PerCP-conjugated Antibody
Product Specific Notices for Human c-Myc PerCP-conjugated Antibody
For research use only
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