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Human c-Myc Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3696

R&D Systems, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Knockout Validated, Simple Western, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 9E10

Product Specifications

Immunogen

C-terminal region peptide of human c-Myc
Ala408-Ala439
Accession # P01106

Specificity

Detects endogenous human c-Myc and c-Myc tagged proteins in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human c-Myc Antibody

Detection of Human c-Myc antibody by Western Blot.

Detection of Human c‑Myc by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human c-Myc Monoclonal Antibody (Catalog # MAB3696) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for c-Myc at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of c-Myc-tagged Protein antibody by Western Blot.

Detection of c‑Myc-tagged Protein by Western Blot.

Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected (-) or transfected with c-Myc-tagged recombinant mouse Wnt-3a. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human c-Myc Monoclonal Antibody (Catalog # MAB3696) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for c-Myc-tagged recombinant mouse Wnt-3a at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of c-Myc antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

Detection of c‑Myc in Jurkat Human Cell Line by Flow Cytometry.

Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human c-Myc Mono-clonal Antibody (Catalog # MAB3696, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Applications for Human c-Myc Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with methanol.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate

Immunoprecipitation

Fan H, et al. (1998) Biochem. Cell. Biol. 76:125.

Knockout Validated

c‑Myc is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in c‑Myc knockout HEK293T cell line.

Simple Western

20 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and c-Myc-tagged recombinant human ANGPTL6

Western Blot

2 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and CHO Chinese hamster ovary cell line either transfected with c-Myc-tagged recombinant mouse Wnt-3a
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.3 using MAB3696 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: c-Myc

Human c-Myc is a helix-loop-helix transcription factor which efficiently binds DNA after heterodimerization with the bHLH protein Max.  It is often overexpressed and mutated in hematopoietic tumors. Mutations frequently result in truncation around amino acid (aa) 252, before the C-terminal DNA binding, HLH and leucine zipper domains. The 439 aa human c-Myc has one O-glycosylation site and has three Ser/Thr phosphorylation sites near the N-terminus. Human c-Myc shows 92% aa identity with mouse or rat c-Myc.

 

Long Name

v-Myc Avian Myelocytomatosis Viral Oncogene Homolog (Avian)

Alternate Names

cMyc, Myc, Myc2, Niard, Nird

Entrez Gene IDs

4609 (Human); 17869 (Mouse); 24577 (Rat)

Gene Symbol

MYC

UniProt

Additional c-Myc Products

Product Documents for Human c-Myc Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human c-Myc Antibody

For research use only

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