Human Carbonic Anhydrase I/CA1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2180
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 363121
Product Specifications
Immunogen
E. coli-derived recombinant human Carbonic Anhydrase I/CA1
Ala2-Phe261
Accession # P00915
Ala2-Phe261
Accession # P00915
Specificity
Detects human Carbonic Anhydrase I/CA1 in direct ELISAs and Western blots. In Western blots, 30% cross-reactivity with recombinant human (rh) CA2 is observed and no cross-reactivity with rhCA3, -4, -7, -8, -10, -11, -12, -13, or -14 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human Carbonic Anhydrase I/CA1 Antibody
Detection of Human Carbonic Anhydrase I/CA1 by Western Blot.
Western blot shows lysates of HEL 92.1.7 human erythroleukemic cell line, human liver tissue, and human colon tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Carbonic Anhydrase I/CA1 Monoclonal Antibody (Catalog # MAB2180) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Carbonic Anhydrase I/CA1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Carbonic Anhydrase I/CA1 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: HEL 92.1.7 human erythroleukemic cell line, human liver tissue, and human colon tissue
Sample: HEL 92.1.7 human erythroleukemic cell line, human liver tissue, and human colon tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase I/CA1
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). CA1 is a cytosolic enzyme with the highest levels in erythrocytes and is a very early marker for erythroid differentiation (2). The activity of CA1 can also be measured by its ability to catalyze the reaction CO2 + H2O → HCO3- + H+, using a published method (3).
References
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Sly, W.S. and P.Y. Hu (1995) Annu. Rev. Biochem. 64:375.
- Wilbur, K.M. and N.G. Anderson (1948) J. Biol. Chem. 176:147.
Alternate Names
CA1
Entrez Gene IDs
759 (Human)
Gene Symbol
CA1
UniProt
Additional Carbonic Anhydrase I/CA1 Products
Product Documents for Human Carbonic Anhydrase I/CA1 Antibody
Product Specific Notices for Human Carbonic Anhydrase I/CA1 Antibody
For research use only
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