Human Carbonic Anhydrase III/CA3 Antibody

Name: Human Carbonic Anhydrase III/CA3 Antibody
Brand: R&D Systems
SKU: MAB2185

R&D Systems, part of Bio-Techne | Catalog # MAB2185

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # 495018

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Product Specifications

Immunogen

E. coli-derived recombinant human Carbonic Anhydrase III/CA3
Ala2-Lys260
Accession # P07451

Specificity

Detects human Carbonic Anhydrase III/CA3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CA1, 2, 4, 5a, 5b, 6, 7, 8, or 9 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human Carbonic Anhydrase III/CA3 Antibody

Detection of Human Carbonic Anhydrase III by Western Blot.

Western blot shows lysates of human skeletal muscle tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Carbonic Anhydrase III Monoclonal Antibody (Catalog # MAB2185) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Carbonic Anhydrase III at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Carbonic Anhydrase III/CA3 Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Cell lysates spiked with Recombinant Human Carbonic Anhydrase III (Catalog # 2185-CA), see our available Western blot detection antibodies

Western Blot

2 µg/mL
Sample: Human skeletal muscle tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase III/CA3

Carbonic Anhydrase is a group of metalloproteases that catalyze the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). CA3 is an approximately 29 kDa cytosolic enzyme with a very low CA activity. It is expressed at low levels in human muscle during early development but increases rapidly during the last trimester to reach 50‑60% of adult levels at birth (2). It is present at high levels in skeletal muscle and much lower levels in other tissues, such as cardiac and smooth muscle. The amino acid sequence of human CA3 is 99%, 90% and 92% identical to that of chimpanzee, dog and mouse/rat.

References

Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
Sly, W.S. and P.Y. Hu (1995) Annu. Rev. Biochem. 64:375.

Alternate Names

CA3

Entrez Gene IDs

761 (Human)

Gene Symbol

CA3

UniProt

P07451

Additional Carbonic Anhydrase III/CA3 Products

Product Documents for Human Carbonic Anhydrase III/CA3 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Carbonic Anhydrase III/CA3 Antibody

For research use only

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