Human Carnosine Dipeptidase 1/CNDP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2489
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunoprecipitation, Western Blot
Cited:
ELISA Development
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carnosine Dipeptidase 1/CNDP1
Pro28-His507
Accession # Q96KN2
Pro28-His507
Accession # Q96KN2
Specificity
Detects human Carnosine Dipeptidase 1/CNDP1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Carnosine Dipeptidase 1/CNDP1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed human neural progenitor cells differentiated by growth factor withdrawal
Sample: Immersion fixed human neural progenitor cells differentiated by growth factor withdrawal
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carnosine Dipeptidase 1/CNDP1 (Catalog # 2489-ZN), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Carnosine Dipeptidase 1/CNDP1 (Catalog # 2489-ZN), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human Carnosine Dipeptidase 1/CNDP1 (Catalog # 2489-ZN)
Sample: Recombinant Human Carnosine Dipeptidase 1/CNDP1 (Catalog # 2489-ZN)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carnosine Dipeptidase 1/CNDP1
The human CNDP1 gene encodes carnosine dipeptidase 1, a member of the M20 family of metalloproteases (1, 2). Also known as X-His dipeptidase, glutamate carboxypeptidase-like protein 2 (CPGL-2) or carnosinase 1 (CN1), CNDP1 is a secreted dipeptidase with a narrow specificity for Xaa-His dipeptides including those with Xaa = beta-Ala (carnosine) and Xaa = gamma-aminobutyric acid (homocarnosine), two naturally occurring dipeptides with potential neuroprotective and neurotransmitter fucntions in the brain. In comparison, a closely related protein known as CNDP2, CPGL or CN2, is a cytosolic nonspecific dipeptidase.
References
- Teufel, M. et al. (2004) J. Biol. Chem. 278:6521.
- Bauer, K. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1022, Academic Press, San Diego.
Alternate Names
Carnosinase 1, CNDP1, CPGL2
Entrez Gene IDs
84735 (Human)
Gene Symbol
CNDP1
UniProt
Additional Carnosine Dipeptidase 1/CNDP1 Products
Product Documents for Human Carnosine Dipeptidase 1/CNDP1 Antibody
Product Specific Notices for Human Carnosine Dipeptidase 1/CNDP1 Antibody
For research use only
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