Human Carnosine Dipeptidase 1/CNDP1 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF2489
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
ELISA Development (Detection)
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carnosine Dipeptidase 1/CNDP1
Pro28-His507
Accession # Q96KN2
Pro28-His507
Accession # Q96KN2
Specificity
Detects human Carnosine Dipeptidase 1/CNDP1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Carnosine Dipeptidase 1/CNDP1 Biotinylated Antibody
Detection of Human Carnosine Dipeptidase 1/CNDP1 by ELISA
Identification and validation of CNPD1 reduction in CC.Detected protein levels are shown for A. the screen using Sandwich Bead Arrays (SBA) and B. validation by Sandwich Immunoassays (SIA) and expressed as mean intensity fluorescence (MIF). C. There was an excellent correlation between results obtained by SBA and SIA. CNPD1 levels measured by SIA were not influenced by D. age or E. gender. F. Distribution of circulating CNDP1 concentrations determined by SIA in Cohort 2. WS = Weight stable, CC = Cancer cachexia. P- and rho-values are given. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0123566), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human Carnosine Dipeptidase 1/CNDP1 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Carnosine Dipeptidase 1/CNDP1 (Catalog # 2489-ZN)
Sample: Recombinant Human Carnosine Dipeptidase 1/CNDP1 (Catalog # 2489-ZN)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carnosine Dipeptidase 1/CNDP1
The human CNDP1 gene encodes carnosine dipeptidase 1, a member of the M20 family of metalloproteases (1, 2). Also known as X-His dipeptidase, glutamate carboxypeptidase-like protein 2 (CPGL-2) or carnosinase 1 (CN1), CNDP1 is a secreted dipeptidase with a narrow specificity for Xaa-His dipeptides including those with Xaa = beta-Ala (carnosine) and Xaa = gamma-aminobutyric acid (homocarnosine), two naturally occurring dipeptides with potential neuroprotective and neurotransmitter fucntions in the brain. In comparison, a closely related protein known as CNDP2, CPGL or CN2, is a cytosolic nonspecific dipeptidase.
References
- Teufel, M. et al. (2004) J. Biol. Chem. 278:6521.
- Bauer, K. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1022, Academic Press, San Diego.
Alternate Names
Carnosinase 1, CNDP1, CPGL2
Entrez Gene IDs
84735 (Human)
Gene Symbol
CNDP1
UniProt
Additional Carnosine Dipeptidase 1/CNDP1 Products
Product Documents for Human Carnosine Dipeptidase 1/CNDP1 Biotinylated Antibody
Product Specific Notices for Human Carnosine Dipeptidase 1/CNDP1 Biotinylated Antibody
For research use only
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