Human Caspase-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB707
Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Knockout Validated, Western Blot
Cited:
ELISA-Based Protein Pin Array, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 84803
Product Specifications
Immunogen
E. coli-derived recombinant human Caspase-3 (18 kDa subunit)
aa 29-175
Accession # P42574
aa 29-175
Accession # P42574
Specificity
Detects human precursor Caspase-3 and the 18 kDa subunit generated during apoptosis.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Caspase-3 Antibody
Detection of Human Precursor Caspase‑3 and p18 Subunit by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 µM staurosporine (STS) for 3 hours. PVDF membrane was probed with 1 µg/mL of Human Caspase-3 Monoclonal Antibody (Catalog # MAB707), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for precursor Caspase-3 and the p18 subunit at approximately 36 and 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Western Blot Shows Human Caspase‑3 Specificity by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Caspase-3 knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Caspase-3 Monoclonal Antibody (Catalog # MAB707) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Caspase-3 at approximately 32 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Caspase-3 Antibody
Application
Recommended Usage
Knockout Validated
Caspase‑3
is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in
Caspase‑3 knockout HeLa cell line.
Western Blot
1 µg/mL
Sample: Staurosporine-treated Jurkat human acute T cell leukemia cell line
Sample: Staurosporine-treated Jurkat human acute T cell leukemia cell line
Reviewed Applications
Read 1 review rated 5 using MAB707 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caspase-3
Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed, and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme. There is one potential variant that shows an alternative start site nine aa upstream of the standard start site coupled with a 21 aa substitution for aa 162-277. Over aa 29-175, human and mouse caspase-3 share 87% aa identity.
Alternate Names
Apopain, CASP3, Caspase3, CPP32, LICE-1, YAMA
Gene Symbol
CASP3
UniProt
Additional Caspase-3 Products
Product Documents for Human Caspase-3 Antibody
Product Specific Notices for Human Caspase-3 Antibody
For research use only
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