Detects human CCL7/MCP-3/MARC in ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human (rh) CCL2/MCP-1, rhCCL8/MCP‑2, or rhCCL13/MCP‑4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CCL7/MCP-3/MARC Antibody

Chemotaxis Induced by CCL7/MCP-3 and Neutralization by Human CCL7/MCP-3 Antibody.

Recombinant Human CCL7/MCP-3 (Catalog # 282-P3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL7/MCP-3 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL7/MCP-3 Monoclonal Antibody (Catalog # MAB282). The ND₅₀ is typically 1.5-4.5 µg/mL.

Applications for Human CCL7/MCP-3/MARC Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: THP-1 human acute monocytic leukemia cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Human CCL7/MCP-3/MARC (Catalog # 282-P3)

Neutralization

Measured by its ability to neutralize CCL7/MCP-3/MARC-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR2A. The Neutralization Dose (ND₅₀) is typically 1.5-4.5 µg/mL in the presence of 1 µg/mL Recombinant Human CCL7/MCP-3/MARC.

Human CCL7/MCP-3 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Human CCL7/MCP‑3/MARC Biotinylated Antibody (Catalog # BAF282)
Standard: Recombinant Human CCL7/MCP-3 Protein (Catalog # 282-P3)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL7/MCP-3/MARC

MCP-2 and CCL7 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and CCL7 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. CCL7 also shares 58% amino acid identity with MCP-2.

CCL7 cDNA encodes a 99 amino acid residue precursor protein from which the N-terminal 23 amino acid residues are cleaved to generate the 76 amino acid residue mature CCL7. Mature CCL7 contains a potential N-linked and several possible O-linked glycosylation sites.

Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4⁺ and CD8⁺ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Alternate Names

MARC, MCP-3

Entrez Gene IDs

6354 (Human); 20306 (Mouse)

Gene Symbol

CCL7

UniProt

Q7Z7Q8

Additional CCL7/MCP-3/MARC Products

Product Documents for Human CCL7/MCP-3/MARC Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CCL7/MCP-3/MARC Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CCL7/MCP-3/MARC Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB282

Key Product Details

Species Reactivity

Validated:

Cited:

Applications