Detects human CCR5. Specifically stains human CCR5 transfectants but not the parental cell lines. It does not cross-react with human CCR1, CCR2, or CCR3. Can be used to detect CCR5 present on stimulated human PBMCs. For additional information regarding epitope specificity for this antibody and other R&D Systems anti-human CCR5 antibodies, see Lee, B. et al. (1999) J. Biol. Chem. 274:9617.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Applications for Human CCR5 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: NS0 mouse myeloma cell line transfected with human CCR5

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR5

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.

Alternate Names

CCR5, CD195

Entrez Gene IDs

1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)

Gene Symbol

CCR5

UniProt

P51681

Additional CCR5 Products

Product Documents for Human CCR5 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CCR5 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CCR5 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB184

Key Product Details

Species Reactivity

Validated:

Cited:

Applications