Human CD11b/Integrin alpha M Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB16992
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Rat
Applications
Validated:
Immunohistochemistry, Multiplex Immunofluorescence, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 238439
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD11b/Integrin alphaM
Phe17-Asn1105
Accession # NP_001139280
Phe17-Asn1105
Accession # NP_001139280
Specificity
Detects human CD11b/Integrin alphaM in direct ELISAs and Western blots. In Western blots no cross-reactivity with recombinant mouse (rm) Integrins alpha2, alpha3, alpha4, alphaE or rhIntegrins alpha5, alphaV, beta1, beta2, beta3, or beta6.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD11b/Integrin alpha M Antibody
Detection of CD11b/Integrin alpha M in Human Colon Cancer via seqIF™ staining on COMET™
CD11b/Integrin alpha M Antibody was detected in immersion fixed paraffin-embedded sections of human colon cancer using Mouse Anti-Human CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB16992) at 15ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
Detection of CD11b/Integrin alpha M in Human Spleen via Multiplex Immunofluorescence staining on COMET™
CD11b/Integrin alpha M was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human CD11b/Integrin alpha M Monoclonal Antibody (MAB16992) at 15 μg/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
Detection of CD11b/Integrin alpha M in Human Tonsil via seqIF™ staining on COMET™
CD11b/Integrin alpha M Antibody was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB16992) at 15ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.Applications for Human CD11b/Integrin alpha M Antibody
Application
Recommended Usage
Immunohistochemistry
3-25 µg/mL
Sample: Human Spleen Tissue
Sample: Human Spleen Tissue
Multiplex Immunofluorescence
15 µg/mL
Sample: Immersion fixed paraffin embedded human spleen, human tonsil, and human colon cancer tissue
Sample: Immersion fixed paraffin embedded human spleen, human tonsil, and human colon cancer tissue
Western Blot
1 µg/mL
Sample: Recombinant Human Integrin alphaM/CD11b
Sample: Recombinant Human Integrin alphaM/CD11b
Reviewed Applications
Read 1 review rated 5 using MAB16992 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD11b/Integrin alpha M
Alternate Names
CD11b, Integrin alpha M, ITGAM
Gene Symbol
ITGAM
UniProt
Additional CD11b/Integrin alpha M Products
Product Documents for Human CD11b/Integrin alpha M Antibody
Product Specific Notices for Human CD11b/Integrin alpha M Antibody
For research use only
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