Human PBMC were stained with (A) Mouse Anti-Human CD302/CLEC13A Monoclonal Antibody (Catalog # MAB6367) or (B) isotype control antibody (Catalog # MAB002), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and PE-conjugated Mouse anti-Human CD14 Monoclonal Antibody (Catalog # FAB3832P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD302/CLEC13A Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood monocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD302/CLEC13A

CD302 (also DCL-1 and CLEC13A) is a 31-36 kDa member of the C-type lectin domain family of proteins. It is expressed on cells involved in phagocytosis, including neutrophils, monocytes, dendritic cells and macrophages. CD302 is suggested to facilitate phagocytosis, and participate in cell adhesion. Mature human CD302 is a 210 amino acid (aa) type I transmembrane glycoprotein. It contains a 146 aa extracellular domain (ECD) (aa 23-168) plus a 43 aa cytoplasmic region. The ECD possesses a C-type lectin domain (aa 32-152) that apparently cannot bind carbohydrate. CD302 has also been detected in dendritic cells as the C-terminal portion of a 215 kDa fusion protein that involves DEC205. CD302 contributes aa 23-232 to the fusion protein. There is one potential alternative start site at Met63. Over aa 23-168, human CD302 shares 82% aa identity with mouse CD302.

Alternate Names

BIMLEC, CD302, CLEC13A, DCL1

Entrez Gene IDs

9936 (Human); 66205 (Mouse); 295629 (Rat)

Gene Symbol

CD302

UniProt

Q81X05

Additional CD302/CLEC13A Products

Product Documents for Human CD302/CLEC13A Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD302/CLEC13A Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CD302/CLEC13A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6367

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CD302/CLEC13A Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CD302/CLEC13A Antibody

Detection of CD302/CLEC13A in Human Monocytes by Flow Cytometry.