Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Porcine, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Knockout Validated, Western Blot

Cited:

Bioassay, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 9G11

View all CD31/PECAM-1 Antibodies »

Product Specifications

Immunogen

Activated HUVEC human umbilical vein endothelial cells

Specificity

Detects human CD31/PEACAM-1. In Western blots, no cross-reactivity with recombinant human (rh) E-Selectin, rhICAM-1, -2, -3, rhVCAM‑1, or recombinant mouse VCAM‑1 was observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD31/PECAM-1 Antibody

Detection of Human CD31/PECAM-1 antibody by Western Blot.

Detection of Human CD31/PECAM‑1 by Western Blot.

Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CD31/PECAM-1 Monoclonal Antibody (Catalog # BBA7) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CD31/PECAM-1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CD31/PECAM-1 antibody in HUVEC Human Cells by Immunocytochemistry (ICC).

CD31/PECAM-1 in HUVEC Human Cells.

CD31/PECAM-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using 10 µg/mL Mouse Anti-Human CD31/PECAM-1 Monoclonal Antibody (Catalog # BBA7) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

CD31/PECAM-1 Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

CD31/PECAM-1 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line but is not detected in CD31/PECAM-1 knockout (KO) THP-1 human cell line using Mouse Anti-Human CD31/PECAM-1 Monoclonal Antibody (Catalog # BBA7) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Applications for Human CD31/PECAM-1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Artery in Human Liver

Immunoprecipitation

Lampugnani, M.G. et al. (1992) J. Cell Biol. 118:1511.

Knockout Validated

CD31/PECAM‑1 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line but is not detected in CD31/PECAM‑1 knockout (KO) THP‑1 human cell line.

Western Blot

1 µg/mL
Sample: HUVEC human umbilical vein endothelial cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 7 reviews rated 4.1 using BBA7 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD31/PECAM-1

The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alphav beta3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Alternate Names

CD31, EndoCAM, PECA1, PECAM-1, PECAM1

Entrez Gene IDs

5175 (Human); 18613 (Mouse); 29583 (Rat)

Gene Symbol

PECAM1

Additional CD31/PECAM-1 Products

Product Documents for Human CD31/PECAM-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD31/PECAM-1 Antibody

For research use only

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