Detects human CD31/PECAM‑1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) E‑Selectin, rhICAM-1, -2, -3, rhVCAM-1, or recombinant mouse VCAM-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human CD31/PECAM-1 APC-conjugated Antibody

Detection of CD31/PECAM‑1 in Human Granulocytes by Flow Cytometry.

Human peripheral blood granulocytes were stained with Mouse Anti-Human CD31/PECAM-1 APC-conjugated Monoclonal Antibody (Catalog # FAB3567A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of CD31/PECAM-1 in THP-1 Human Cell Line by Flow Cytometry.

THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CD31/PECAM-1 APC-conjugated Monoclonal Antibody (Catalog # FAB3567A, filled histogram) or isotype control antibody (IC002A, open histogram). Staining was performed using our Membrane-Associated Staining protocol.

CD31/PECAM-1 Specificity is Shown by Flow Cytometry in Knockout Cell Line.

CD31/PECAM-1 knockout THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human CD31/PECAM-1 APC-conjugated Monoclonal Antibody (Catalog # FAB3567A, filled histogram) or isotype control antibody (IC002A, open histogram). No staining in the CD31/PECAM-1 knockout THP-1 cell line was observed. Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human CD31/PECAM-1 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood granulocytes and THP-1 acute monocytic leukemia cell line

Knockout Validated

CD31/PECAM-1 is specifically detected in THP-1 myelogenous leukemia parental cell line but is not detectable in CD31/PECAM-1 knockout THP-1 cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using FAB3567A in the following applications:

Immunocytochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD31/PECAM-1

The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alphav beta3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Alternate Names

CD31, EndoCAM, PECA1, PECAM-1, PECAM1

Entrez Gene IDs

5175 (Human); 18613 (Mouse); 29583 (Rat)

Gene Symbol

PECAM1

Additional CD31/PECAM-1 Products

Product Documents for Human CD31/PECAM-1 APC-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human CD31/PECAM-1 APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human CD31/PECAM-1 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB3567A

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications