Human CD34 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7227P

R&D Systems, part of Bio-Techne
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FAB7227P
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Canine

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # QBEnd10

View all CD34 Antibodies »

Product Specifications

Immunogen

Human endothelial vesicles

Specificity

Detects human CD34 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD34 PE-conjugated Antibody

Detection of CD34 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CD34 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD34 PE-conjugated Monoclonal Antibody (Catalog # FAB7227P) and Mouse Anti-Human CD45 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB1430N). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
Detection of CD34 antibody in KG-1a Human Cell Line antibody by Flow Cytometry.

Detection of CD34 in KG-1a Human Cell Line by Flow Cytometry.

KG-1a human acute myelogenous leukemia cell line was stained with Mouse Anti-Human CD34 PE-conjugated Monoclonal Antibody (Catalog # FAB7227P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD34 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) and KG‑1a human acute myelogenous leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD34

The CD34 family of cell surface transmembrane proteins includes the hematopoietic progenitor cell antigen CD34, Podocalyxin, and Endoglycan. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein kinase C (PKC). CD34 is a 115 kDa glycoprotein found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines. CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.

Alternate Names

CD34, HPCA1

Entrez Gene IDs

947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)

Gene Symbol

CD34

Additional CD34 Products

Product Documents for Human CD34 PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD34 PE-conjugated Antibody

For research use only

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