Human CD63 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB50481
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2585C
Product Specifications
Immunogen
Chinese Hamster Ovary cell line CHO-derived human CD63
Ala63-Val203
Accession # P08962-1
Ala63-Val203
Accession # P08962-1
Specificity
Detects human CD63 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human CD63 Antibody
Detection of CD63 in Human PBMC by Flow Cytometry.
Human PBMC were stained with Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50481, filled histogram) or isotype control antibody (MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.
CD63 in A431 Human Cell Line.
CD63 was detected in immersion fixed A431 human epithelial carcinoma cell line using Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50481) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of CD63 in PBMC monocytes by Flow Cytometry
PBMC monocytes were stained with Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50481, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins.Applications for Human CD63 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human PBMCs fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012); PBMC monocytes (surface stain)
Sample: Human PBMCs fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012); PBMC monocytes (surface stain)
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line
Sample: Immersion fixed A431 human epithelial carcinoma cell line
Reviewed Applications
Read 1 review rated 5 using MAB50481 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD63
Alternate Names
CD63, Granulophysin, Lamp-3, ME491, OMA81H, Tspan30
Gene Symbol
CD63
UniProt
Additional CD63 Products
Product Documents for Human CD63 Antibody
Product Specific Notices for Human CD63 Antibody
For research use only
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