| Specificity: |
Detects recombinant human CD8 in direct ELISA
Applications for Human CD8 Antibody
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Recommended Concentration
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Sample |
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Cell depletion |
2 µg/mL |
Selects CD8+ cells when immobilized to cell culture plate or bead |
Scientific Data Examples for Human CD8 Antibody
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Cell Depletion
Immobilized recombinant CD8 antibody supports the adhesion of SUP-T1 cells to cell culture plates.
The CD8 antibody was coated onto cell culture plates at increasing amounts. The wells were then incubated with calcein labeled SUP-T1 cells, and cell binding was detected using a fluorescence-based plate reader. The ED50 for this effect is 0.200‑2.00 μg/mL.
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Formulation, Preparation and Storage
| Purification |
Protein G purified from cell culture supernatant
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| Reconstitution |
Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
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| Formulation |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.
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| Shipping |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
*Small pack size (-SP) is shipped at ambient temperature or with polar packs. Upon receipt, store it immediately at the temperature
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| Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator
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Product Documents for Human CD8 Antibody
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