Human whole blood granulocytes were stained with Mouse Anti-Human CD82/Kai-1 Monoclonal Antibody (Catalog # MAB4616, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).

Applications for Human CD82/Kai-1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human whole blood granulocytes

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4.3 using MAB4616 in the following applications:

ELISA (1 Review)
Microarray (1 Review)
Microarrays (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD82/Kai-1

CD82, also known as KAI1, C33 and TSPAN27, is a widely expressed palmitoylated molecule in the tetraspanin superfamily. CD82 is synthesized as a 28 kDa core protein with mature molecular weights ranging up to 70 kDa due to variable glycosylation. CD82 functions as a suppressor of metastasis and is down‑regulated in many malignant tumors. It associates with IGSF8, DARC, the ganglioside GM2, and the HTLV-1 Gag protein. CD82 inhibits cell migration and metastasis by preventing integrin maturation and blocking integrin interactions with Met. Human CD82 shares 76% aa sequence identity with mouse and rat CD82.

Alternate Names

CD82, IA4, KAI1, SAR2, ST6, TSPAN27

Entrez Gene IDs

3732 (Human); 12521 (Mouse); 83628 (Rat)

Gene Symbol

CD82

UniProt

AAH01821

Additional CD82/Kai-1 Products

Product Documents for Human CD82/Kai-1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD82/Kai-1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CD82/Kai-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4616

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CD82/Kai-1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CD82/Kai-1 Antibody

Detection of CD82/Kai-1 in Human Granulocytes by Flow Cytometry.