Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD8 alpha Monoclonal Antibody (Catalog # MAB3803) or (B) Mouse IgG2A isotype control antibody (MAB003) followed by anti-Mouse IgG Phycoerythrin-conjugated secondary antibody (F0102B) and Mouse Anti-Human CD3 epsilon Allophycocyanin-conjugated Monoclonal Antibody (FAB100A). Staining was performed using our Staining Membrane-associated Proteins protocol.

Detection of CD8 alpha in HEK293 Human Cell Line Transfected with Cynomolgous Monkey CD8 alpha and eGFP by Flow Cytometry

HEK293 human embryonic kidney cell line transfected with (A) cynomolgous monkey CD8 alpha or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human/Cynomolgous Monkey CD8 alpha Monoclonal Antibody (Catalog # MAB3803) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB003) .Staining was done using our Staining Membrane-associated Proteins protocol.

CD8 alpha in Human PBMCs.

CD8 alpha was detected in immersion fixed Human peripheral blood mononuclear cells (PBMCs, positive staining) and HDLM-2 human Hodgkin’s lymphoma cell line (negative control) using Mouse Anti-Human CD8 alpha Monoclonal Antibody (Catalog # MAB3803) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Applications for Human CD8 alpha Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human PBMC lymphocytes and HEK293 Human Cell Line Transfected with Cynomolgous Monkey CD8 alpha and eGFP

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Human peripheral blood mononuclear cells (PBMCs)

Immunohistochemistry

0.5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymph node

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB3803 in the following applications:

Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD8

CD8 is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes.

Alternate Names

CD8, CD8A

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Gene Symbol

CD8A

UniProt

NP_741969.1

Additional CD8 Products

Product Documents for Human CD8 alpha Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD8 alpha Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CD8 alpha Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3803

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CD8 alpha Antibody

Detection of CD8 alpha in Human Blood Lymphocytes by Flow Cytometry.