Human CD8 alpha Fluorescein-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB1509F
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry, Immunocytochemistry
Label
Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 37006
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD8 alpha
Ser22-Val198
Accession # NP_741969
Ser22-Val198
Accession # NP_741969
Specificity
Detects human CD8 alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with mouse CD8 alpha is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human CD8 alpha Fluorescein-conjugated Antibody
Detection of CD8 alpha in Human Blood Lymphocytes by Flow Cytometry.
Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD8a Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1509F) or (B) Mouse IgG2B isotype control antibody (Catalog # IC0041F) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). View our protocol for Staining Membrane-associated Proteins.Applications for Human CD8 alpha Fluorescein-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood lymphocytes
Sample: Human peripheral blood lymphocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD8
CD8 is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes.
Alternate Names
CD8, CD8A
Entrez Gene IDs
Gene Symbol
CD8A
UniProt
Additional CD8 Products
Product Documents for Human CD8 alpha Fluorescein-conjugated Antibody
Product Specific Notices for Human CD8 alpha Fluorescein-conjugated Antibody
For research use only
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