Human whole blood monocytes were stained with Mouse Anti-Human IL-3 R alpha/CD123 PE-conjugated Monoclonal Antibody (FAB301P) and either (A) Mouse Anti-Human ChemR23 APC-conjugated Monoclonal Antibody (Catalog # FAB362A) or (B) Mouse IgG₃Allophycocyanin Isotype Control (IC007A). View our protocol for Staining Membrane-associated Proteins.

Detection of ChemR23 in HEK293 Human Cell Line Transfected with Human ChemR23 and eGFP by Flow Cytometry.

HEK293 human cell line (A) transfected with human ChemR23 or (B) irrelevant protein and eGFP, were stained with Mouse Anti-Human ChemR23 APC-conjugated Monoclonal Antibody (Catalog # FAB362A). Quadrant markers were set based on Mouse IgG3Allophycocyanin Isotype Control (IC007A, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human ChemR23 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human whole blood monocytes and HEK293 Human Cell Line Transfected with Human ChemR23 and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: ChemR23

ChemR23, also known as CMKLR1, is a 47-48 kDa 7-transmembrane member of the rhodopsin, or G-protein coupled receptor family 1. It has restricted expression on select cell types that, in primate, include adipocytes, astrocytes, endothelial cells, microglia/macrophages, chondrocytes, monocytes, osteocytes, NK cells, and plasmacytoid plus myeloid dendritic cells. In mouse, would studies show ChemR23 to be expressed by neutrophils and keratinocytes. The universally-agreed on ligand for ChemR23 is Chemerin, a 137 amino acid (aa) non-chemokine product of fibroblasts, platelets, adipocytes and endothelium. The Chermerin:ChemR23 system is suggested to promote chemotaxis for all ChemR23+ cells, and to induce angiogenesis via endothelial cell migration and MMP-2 and -9 activation. There is one alternative splice form that shows a deletion of aa 1 and 2 (NP_004063). Over the extracellular domains of ChemR23, human and mouse share 69% aa sequence identity.

Long Name

Chemokine-like Receptor 1/Orphan G Protein-coupled Receptor ChemR23

Alternate Names

cmklr1, Dez, Gpcr27

Entrez Gene IDs

1240 (Human); 14747 (Mouse); 60669 (Rat)

Gene Symbol

CMKLR1

UniProt

NP_004063

Additional ChemR23 Products

Product Documents for Human ChemR23 APC-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human ChemR23 APC-conjugated Antibody

For research use only

Citations
Reviews

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Flow Cytometry

Human ChemR23 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB362A

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all ChemR23 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human ChemR23 APC-conjugated Antibody

Detection of ChemR23 in Human Whole Blood Monocytes by Flow Cytometry.