Human peripheral blood monocytes were stained with Mouse Anti-Human IL-3 Ra/CD123 PerCP-conjugated Monoclonal Antibody (FAB301C) and either (A) Mouse Anti-Human ChemR23 PE-conjugated Monoclonal Antibody (Catalog # FAB362P) or (B) Mouse IgG₃Phycoerythrin Isotype Control (IC007P). View our protocol for Staining Membrane-associated Proteins.

Detection of ChemR23 in HEK293 Human Cell Line Transfected with Human ChemR23 and eGFP by Flow Cytometry.

HEK293 human cell line (A) transfected with human ChemR23 or (B) irrelevant protein and eGFP, were stained with Mouse Anti-Human ChemR23 PE-conjugated Monoclonal Antibody (Catalog # FAB362P). Quadrant markers were set based on Mouse IgG3 PE Isotype Control (IC007P, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human ChemR23 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood monocytes and HEK293 Human Cell Line Transfected with Human ChemR23 and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: ChemR23

ChemR23 is a chemoattractant receptor expressed on dendritic cells and activated macrophages. ChemR23 binds Chemerin and functions as a coreceptor for SIV and some primary HIV-1 strains.

References

Samson, M. et al. (1998) Eur. J. Immunol. 28:1689.

Long Name

Chemokine-like Receptor 1/Orphan G Protein-coupled Receptor ChemR23

Alternate Names

cmklr1, Dez, Gpcr27

Entrez Gene IDs

1240 (Human); 14747 (Mouse); 60669 (Rat)

Gene Symbol

CMKLR1

UniProt

NP_004063

Additional ChemR23 Products

Product Documents for Human ChemR23 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ChemR23 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human ChemR23 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB362P

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all ChemR23 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human ChemR23 PE-conjugated Antibody

Detection of ChemR23 in Human Blood Monocytes by Flow Cytometry.