Human cIAP-1/HIAP-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB818
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Knockout Validated, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 681732
Product Specifications
Immunogen
E. coli-derived recombinant human cIAP-1/HIAP-2
His2-Ser618
Accession # Q13490
His2-Ser618
Accession # Q13490
Specificity
Detects human cIAP‑1/HIAP-2 in direct ELISAs and Western blots.
In direct ELISAs, 100% cross‑reactivity
with recombinant human (rh) cIAP-2 (aa 2‑604) and no cross-reactivity with
rhBIRC6 (aa 4582‑4735), rhcIAP‑2 (aa 94‑178), rhXIAP (aa 1‑497), rhXIAP
(BIR2 domain; aa 124‑242), or rhXIAP (BIR3 domain; aa 252‑356) is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human cIAP-1/HIAP-2 Antibody
Detection of Human cIAP-1/HIAP-2 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected, transfected with full length human cIAP-1, or transfected with full length human cIAP-2, HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human cIAP-1/HIAP-2 Monoclonal Antibody (Catalog # MAB818) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for cIAP-1/HIAP-2 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human cIAP‑1/HIAP‑2 by Simple WesternTM.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for cIAP‑1/HIAP‑2 at approximately 66 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human cIAP‑1/HIAP‑2 Monoclonal Antibody (Catalog # MAB818) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western Blot Shows Human cIAP-1/HIAP-2 Specificity by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and cIAP-1/HIAP-2 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human cIAP-1/HIAP-2 Monoclonal Antibody (Catalog # MAB818) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for cIAP-1/HIAP-2 at approximately 68 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human cIAP-1/HIAP-2 Antibody
Application
Recommended Usage
Knockout Validated
cIAP‑1/HIAP‑2
is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in
cIAP‑1/HIAP‑2 knockout HeLa cell line.
Simple Western
5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Western Blot
1 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with full length human cIAP-1, or transfected with full length human cIAP-2, HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and A431 human epithelial carcinoma cell line
Sample: HEK293 human embryonic kidney cell line transfected with full length human cIAP-1, or transfected with full length human cIAP-2, HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and A431 human epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using MAB818 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: cIAP-1/HIAP-2
cIAP-1 (also known as BIR2, MIHB and HIAP-2) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. cIAP-1 has 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-1 inhibits caspases by interaction of the BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HTRA-2/Omi. cIAP-1 is reported to be cleaved by caspases in fetal rat hepatocytes treated with TGF-beta.
References
- Roy, N. et al. (1997) EMBO J. 23:6914.
- Deveraux, Q. et al. (1997) Nature 388:300.
- Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239.
- Herrera, B. et al. (2002) FEBS Letters 520:93.
Long Name
Cellular Inhibitor of Apoptosis Protein 1
Alternate Names
BIRC2, cIAP1, HIAP-2, MIHB
Gene Symbol
BIRC2
UniProt
Additional cIAP-1/HIAP-2 Products
Product Documents for Human cIAP-1/HIAP-2 Antibody
Product Specific Notices for Human cIAP-1/HIAP-2 Antibody
For research use only
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