Human CILP-1 N-Terminal Fragment Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5504
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CILP‑1 N-Terminal Fragment
Arg22-Arg720
Accession # NP_003604
Arg22-Arg720
Accession # NP_003604
Specificity
Detects human CILP‑1 N-Terminal Fragment in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CILP-1 C-terminal peptide is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human CILP-1 N-Terminal Fragment Antibody
Detection of Human CILP‑1 N-Terminal Fragment by Western Blot.
Western blot shows lysates of human cartilage tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CILP-1 N-Terminal Fragment Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5504) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CILP-1 at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human CILP-1 by Simple WesternTM.
Simple Western lane view shows lysates of human cartilage tissue, loaded at 0.2 mg/mL. A specific band was detected for CILP-1 at approximately 133 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CILP-1 N-Terminal Fragment Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5504) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.Applications for Human CILP-1 N-Terminal Fragment Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: Human cartilage tissue
Sample: Human cartilage tissue
Western Blot
1 µg/mL
Sample: Human cartilage tissue
Sample: Human cartilage tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CILP-1
The CILP-1 (cartilage intermediate-layer protein 1) gene product is a 132 kDa (predicted) monomeric glycoprotein that is found in both hyaline and fibrocartilage. It is a precursor for two secreted, proteolytically generated products, a 90 kDa N-terminal CILP-1, and a 62 kDa C-terminal NTPPHase-homolog. The N-terminus is suggested to serve as both a matrix structural protein, and an IGF-I/TGF-beta 1 suppressor sequestration molecule. Human CILP-1 spans aa 22‑720 of the CILP-1 precursor. It contains one TSP-1 domain (aa 149‑201), a C2‑type Ig-like region (aa 309‑395) and six potential N-glycosylation sites. Over aa 1‑720 of the CILP-1 precursor, human CILP-1 shares 89% aa identity with mouse CILP‑1, and 42% aa identity with human CILP-2.
Long Name
Cartilage Intermediate Layer Protein 1
Alternate Names
CILP, CILP1
Gene Symbol
CILP
UniProt
Additional CILP-1 Products
Product Documents for Human CILP-1 N-Terminal Fragment Antibody
Product Specific Notices for Human CILP-1 N-Terminal Fragment Antibody
For research use only
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