Human Claspin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3310
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 485508
Product Specifications
Immunogen
E. coli-derived recombinant human Claspin
Phe1138-Ser1339
Accession # Q9HAW4
Phe1138-Ser1339
Accession # Q9HAW4
Specificity
Detects endogenous human Claspin in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Claspin Antibody
Detection of Human Claspin by Western Blot.
Western blot shows lysates of HCT-116 human colorectal carcinoma cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Human Claspin Monoclonal Antibody (Catalog # MAB3310) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Claspin at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Claspin Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HCT-116 human colorectal carcinoma cell line and HEK293 human embryonic kidney cell line
Sample: HCT-116 human colorectal carcinoma cell line and HEK293 human embryonic kidney cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Claspin
Claspin is a nuclear protein whose expression peaks during S and G2 phases of the cell cycle. Claspin is phosphorylated by ATR in response to DNA damage or replication stress. It functions in cell cycle checkpoint control by regulating the activation of Chk1 and BRCA1. Claspin contains three CKB motifs and two coiled coil regions. Over the range used for immunization, human and mouse Claspin share 72% amino acid sequence identity. DNA replication and DNA damage induce the phosphorylation of Claspin by Chk1-dependent mechanisms.
Alternate Names
CLSPN
Gene Symbol
CLSPN
UniProt
Additional Claspin Products
Product Documents for Human Claspin Antibody
Product Specific Notices for Human Claspin Antibody
For research use only
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