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Human Claudin-1 Alexa Fluor® 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB4618R

R&D Systems, part of Bio-Techne
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FAB4618R-100UG

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 421203

Product Specifications

Immunogen

CHO Chinese hamster ovary cell line transfected with human Claudin-1
Met1-Val211
Accession # O95832

Specificity

Stains human Claudin-1 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human Claudin-1 Alexa Fluor® 647-conjugated Antibody

Detection of Claudin-1 antibody in PC-3 Human Cell Line antibody by Flow Cytometry.

Detection of Claudin‑1 in PC‑3 Human Cell Line by Flow Cytometry.

PC-3 human prostate cancer cell line was stained with Rat Anti-Human Claudin-1 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB4618R, filled histogram) or isotype control antibody (Catalog # IC006R, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Claudin-1 Alexa Fluor® 647-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: PC-3 human prostate cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Claudin-1

Claudin-1 is a 23 kDa multipass membrane protein in the Claudin family of epithelial tight junction proteins. Claudin-1 is expressed by epithelial cells in a wide variety of tissues as well as in Langerhans cells and dendritic cells. It is up or downregulated in many cancers and is required for the entry of hepatitis C virus into hepatocytes. Human Claudin-1 shares 91% aa sequence identity with mouse and rat Claudin-1.

Alternate Names

Claudin1, CLD1, CLDN1, SEMP1

Entrez Gene IDs

9076 (Human); 12737 (Mouse); 65129 (Rat)

Gene Symbol

CLDN1

UniProt

Additional Claudin-1 Products

Product Documents for Human Claudin-1 Alexa Fluor® 647-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Claudin-1 Alexa Fluor® 647-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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