HEK293 human embryonic kidney cell line transfected with human Claudin-8 was stained with Mouse Anti-Human Claudin-8 Monoclonal Antibody (Catalog # MAB5275, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Detection of Claudin‑8 in Caki‑2 Human Cell Line by Flow Cytometry.

Caki-2 human clear cell carcinoma epithelial cell line was stained with Mouse Anti-Human Claudin-8 Monoclonal Antibody (Catalog # MAB5275, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human Claudin-8 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human Claudin-8

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: Caki‑2 human clear cell carcinoma epithelial cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Claudin-8

Claudin-8 is a 225 amino acid (aa), 25 kDa four-transmembrane protein in the Claudin family of tight junction proteins. It is selectively expressed in tight junctions in the kidney thin descending limb, distal tubule and collecting duct, but is also found in liver and intestinal tight junctions. It is thought to function as an ion barrier protein. The extracellular portions of human Claudin-8 share 86% and 89% aa identity with corresponding portions of mouse and rat Claudin-8, respectively.

Alternate Names

Claudin8, CLDN8

Entrez Gene IDs

9073 (Human); 54420 (Mouse); 304124 (Rat)

Gene Symbol

CLDN8

UniProt

P56748

Additional Claudin-8 Products

Product Documents for Human Claudin-8 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Claudin-8 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Claudin-8 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5275

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Claudin-8 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Claudin-8 Antibody

Detection of Claudin‑8 in HEK293 Human Cell Line Transfected with Human Claudin-8 by Flow Cytometry.